LymFilter

Maeda H, Okamoto K, Oba K, Shiga M, Fujieda Y, Namikawa T, Hiroi M, Murakami I, Hanazaki K, Kobayashi M.
Lymph node retrieval after dissolution of surrounding adipose tissue for pathological examination of colorectal cancer.
‍Oncol Lett. 2018 Feb;15(2):2495-2500. doi: 10.3892/ol.2017.7629. Epub 2017 Dec 14. PMID: 29434964; PMCID: PMC5777345.

“applying fat dissolution liquid to the remnant adipose tissue of the mesentery of the colon and rectum identified additional LNs. This method should be considered when insufficient LNs are identified after conventional LN retrieval.”

Yu N, Liu H, Li J, Chen S.
Using low concentration sodium hypochlorite to improve colorectal surgical specimen lymph node harvest.
Mol Clin Oncol. 2020 Jun;12(6):519-524. doi: 10.3892/mco.2020.2018. Epub 2020 Mar 19. PMID: 32337032; PMCID: PMC7179393.

“Using sodium hypochlorite at low concentrations may be the most simple, rapid, cost-saving, nontoxic and effective technique to improve LN harvest in colorectal carcinoma specimens so far. This method should be used routinely regardless of whether the number of LNs is <12 or not.”

Vogel C, Kirtil T, Oellig F, Stolte M.
Lymph node preparation in resected colorectal carcinoma specimens employing the acetone clearing method.
Pathol Res Pract. 2008;204(1):11-5. doi: 10.1016/j.prp.2007.08.015. Epub 2007 Nov 19. PMID: 18023994.

“Acetone clearance is an appropriate option to identify accessory lymph nodes in colorectal carcinoma specimens for daily routine use, and also constitutes an excellent instrument for internal quality control.”

Gehoff A, Basten O, Sprenger T, Conradi LC, Bismarck C, Bandorski D, Merkelbach-Bruse S, Schneider-Stock R, Stoehr R, Wirtz RM, Kitz J, Müller A, Hartmann A, Becker H, Ghadimi BM, Liersch T, Rüschoff J.
Optimal lymph node harvest in rectal cancer (UICC stages II and III) after preoperative 5-FU-based radiochemotherapy. Acetone compression is a new and highly efficient method.
Am J Surg Pathol. 2012 Feb;36(2):202-13. doi: 10.1097/PAS.0b013e31823fa35b. PMID: 22251939.

“The AC method increased LN retrieval 4- to-6-fold as compared with the literature and 2-fold compared with manual dissection after the FC method.”

Brown HG, Luckasevic TM, Medich DS, Celebrezze JP, Jones SM.
Efficacy of manual dissection of lymph nodes in colon cancer resections.
Mod Pathol. 2004 Apr;17(4):402-6. doi: 10.1038/modpathol.3800071. PMID: 14976530.

“Although manual lymph node dissection is the current standard at most institutions, recent statistical studies indicate that all lymph nodes, including those measuring 1-2 mm, should be recovered to be assured of lymph node negative status.”

Basten O, Bandorski D, Bismarck C, Neumann K, Fisseler-Eckhoff A.
Acetonkompression. Schnelle, standardisierte Methode zur Untersuchung von Lymphknoten im Gastrointestinaltrakt [Acetone compression. A fast, standardized method to investigate gastrointestinal lymph nodes].
Pathologe. 2010 May;31(3):218-24. German. doi: 10.1007/s00292-009-1256-7. PMID: 20012620.

“ Elution with acetone enables the prompt, standardized and full histological work-up of adipose tissue without manual dissection.”